Biology 26 - Syllabus

Biology 26 - Spring Quarter 2016
Instructor: Doris Spanggord
e-mail address:
Office: SC1218
Telephone/voice mail : (408) 864-8341
Office hrs: MW - 3:30PM -4:30PM

Student Learning Outcomes:

  1. Evaluate and demonstrate the importance of aseptic technique when working with microorganisms
  2. Compare and contrast size, shape, structure, nutritional and environmental requirements of bacteria, viruses, protozoa, and fungi
  3. Investigate host parasite relationships and assess their positive and negative impact on the participants

A. Objectives of the Course:

Lecture periods will be devoted to a consideration of fundamental principles and general activities of microorganisms with special emphasis given to their medical significance and control. Aseptic technique will also be stressed. These will be correlated with laboratory experiments when possible. A schedule for the laboratory exercises is attached. Prerequisites for the course are Biology 40C or its equivalent (a college chemistry and biology course).

B. Required Text and Laboratory Manual:

1. The required text for the lecture portion of the course is, Microbiology, an Introduction, by Tortora, Funke, and Case. Bring the text to class if at all possible.

Students are responsible for reading material in the text as it relates to the various lecture topics. It is assumed that such reading will be to clarify and/or to enhance material given in lecture periods. Examinations will cover lecture material and any questions sets or specific reading assignments given.

2. The required text for the laboratory portion of the course is Tortora and also a laboratory manual (Biology 26 Laboratory Manual, by Doris Spanggord).

The student is expected to read exercises, and answer questions, if any, relevant to that reading before coming to the lab in which that exercise will be performed. Material from the reading in the lab manual is "fair game" for lab quizzes and practicals.

A covering must be worn in the laboratory. This may be an old shirt, smock, or lab coat. These will be kept in the laboratory throughout the course.

3. If possible, purchase a microbiology lab kit costing about $15.99 from the book store. Bring a combination lock or padlock to secure your kit in a lab locker. You can purchase a lock at the book store for about $6.00 or bring one from home

C. Examination and Grading:

1. Lecture and lab will count equally in computing the final course grade. Grading is done by the point system. At the end of the course a percentage of your total number of points is taken for your assigned grade. Determination of borderline grades will be based on attendance and deportment in class.
90-100% - A
80-89% - B
70-79% - C
55-69% - D
below 55% - F
2. The lecture grade will be based on 2 midterms and the final examination. All lecture exams will be comprehensive and will cover general concepts and examples from the laboratory. The laboratory grade will be based on 2 quizzes, an unknown report, and 2 lab exams.

Lecture Exams will be given on (1). Wednesday, April 27th
(2). Wednesday, May 25.

Lecture Final will be given on - We don't know the final schedule for Spring Quarter at this time.

Make-up examinations will NOT BE GIVEN. If you miss an exam, with the exception of the final exams, and have a valid reason for missing the exam,and inform me of the reason for the absence before the exam, your percent in the class will be calculated on the basis of your total number of points at the end of the quarter. If you miss a second test (quiz or exam) you will receive zero points for that exam. If you do not take both final exams (Lab and Lecture), you will fail the course.
If you fail the lecture or lab final exam, you will fail the class.

D. Attendance:
More than two absences from laboratory will result in an automatic drop from the course by the instructor.

The Biological Health Sciences Division Student Handbook is on line available at: as well as in the Science Center Learning Center in hard copy. All the information in the Student Handbook does apply to this course. This handbook contains many helpful resources for students, ideas to increase student success, and expectations of classroom behavior.

E. Additional information:
Labs cannot be made up. Media, reagents, and cultures are available only for the lab for which they were prepared.
Students will not be allowed to leave the room after beginning an examination.
No talking is allowed during lecture. You will be asked to leave the room.


Lab Exercise # /Date to be performed/ Subject

April 4/ Orientation to the Laboratory

1,2/ April 6/ Introduction to Microscopy
Observation of Living Organisms

3,4/ April 11/ Media Preparation
Distribution of microorganisms

5,6/ April 13/ Bacterial Smear/ Simple Stain

7,9/ April 18/ Capsule and Granule Stain
(8 and 10 will be demo's) Acid Fast and Spore Stain

11/ April 20/ The Gram Stain, QUIZ #1

12,13/ April 25/ Identification of Bacterial Unknown
Isolation of Pure Cultures

14,15,16/ April 27/ Aerobic and Anaerobic Culture Tech
Catalase Oxidase Production
Reduction of Nitrates

17,18,19/ May 2/ Carbohydrate Fermentation
Citrate Utilization
Hydrolysis of Starch

20,21,22/ May 4/ Hydrolysis of Gelatin
Motility, Indole, and H2S (SIM agar)
Urease Production

May 9th/ LAB EXAM

May 11/ MR-VP Tests
API 20E Multiple Test Strip,
Selective and Differential Media

26,27/ May 16/ Bacteria of the Throat
Bacteria of the Skin

29,30/ May 18/Viruses (Bacteriophages)

28,40/ February 29 Epidemiology,
The Effect of Hand Washing on Skin Microbiota

33/ May 25/ Effects of Antimicrobials on
Microorganisms - QUIZ #2

34,35,36/ June 1st/ Role of Temperature, and the Effect of Heat, UV light, and Osmotic Pressure (Demo) on Microbial Growth.

June 6th/ Yeasts and Molds

June 8/ Lab

June 13/ Protozoa

June/ LABORATORY FINAL EXAM Unknown bacterial culture report due March 16th.


Last Updated: 4/25/16